Fields of application

Designed to specifically determine the location of certain genes in the sample, these methods are used in most areas of experimental biology and diagnosis. They are largely based on the specific hybridizations of the probes used in the experiments.

This high throughput biology controller designed for ISH/IHC and FISH on in toto samples provides answers to various problems.

In the context of neurobiology, it makes it possible to carry out analyses at the cellular and molecular level to identify the mechanisms involved in the formation of the nervous system but also the modifications made during mutations. It allows experimentation on organs and helps, in particular through the technique of Fluorescence In Situ Hybridization, to highlight different specific neural connections. The use of ISH/IHC and FISH in neurobiology has made it possible to understand the mechanisms of neuronal regeneration and information transmission, but also to develop approaches for the treatment of neurodegenerative diseases.

In oncology, our high throughput biology automaton has made it possible to highlight gene expression in a healthy organism, but especially during tumor formation. Indeed, in situ hybridization and immunohistochemistry techniques make it possible to identify and quantify the genes expressed in a sample. These techniques also allow the implementation of diagnostic protocols for certain cancers whose tumor cells express specific proteins.

The use of the Flo 400 in development biology is becoming obvious. It helps to understand the interactions that occur during organ development and formation and to understand the different diseases that occur during pregnancy. These in situ hybridization and immunohistochemistry techniques make it possible to understand the formation of different organs such as the heart, liver, nervous system, etc. These experiments are carried out on in toto or slide samples which are then analyzed to be able to visualize in 3D the expression of genes in the entire sample.

Research in biology is constantly diversifying and becoming more complex with the emergence of new technologies and the accumulation of knowledge. Thus, different fields of applications and biology experiment are emerging and new high throughput biology techniques are emerging.

In situ hybridization, immunohistochemistry and fluorescence in situ hybridization are tagging methods that provide a clear understanding of the expression of each gene in a spatial and temporal context within a given sample. This allows a characterization of the proteome or genome of the organism studied.

Flogentec thus offers biological automatons that can have several different applications depending on your research field.

The Flo400 is a safe, fast and easy-to-use research device that allows 3D samples to be analyzed using the in situ hybridization and immunohistochemistry methods. Thanks to this equipment, it is possible to directly visualize the location and activation of genes of the cells concerned. The means used for this localization is in situ hybridization (HIS) of RNA probes (transcriptome analysis). It should be noted that the automaton also allows proteome analysis by immunohistochemistry, using antibodies instead of RNA probes.