Transparency of samples

Although different protocols have emerged one after the other in recent years, the complexity of some has made them extremely complicated to use under laboratory conditions, particularly through the use of harmful reagents.

We have studied the use of 6 different protocols to adapt 3 of them to our Flo400 PLC.

This protocol is the shortest since the current total time to perform it on our PLC is 14 hours. After a passage of tetrahydrofuran at increasing concentration in the samples, dichloromethane and di-benzyl ether stabilize the samples until fully transparent samples are obtained. This Protocol makes it possible to preserve the sizes of the embryos but also the fluorescent markings obtained after Immunohistochemistry. Be careful, however, Tetrahydrofuran is a highly toxic reagent and requires handling under a hood. In addition, this compound destroys some plastics such as polypropylene, so you will need to use polystyrene tankers.

These three protocols (SeeDB, 3DISCO and ClearT) have been adapted by our team to allow you to clarify your samples easily and fully automatically. The performance offered by the Flo 400 allows you to clarify more than 50 samples in a single protocol by significantly reducing the amount of reagents required through its recycled flow system.

The Flo400 PLC can also perform the techniques:

Research in biology is constantly diversifying and becoming more complex with the emergence of new technologies and the accumulation of knowledge. Thus, different fields of applications and experiments in biology are emerging and new high throughput biology techniques are emerging.

In situ hybridization, immunohistochemistry and fluorescence in situ hybridization are tagging methods that provide a clear understanding of the expression of each gene in a spatial and temporal context within a given sample. This allows a characterization of the proteome or genome of the organism studied.

Flogentec thus offers biological automatons that can have several different applications depending on your research field.

The Flo400 is a safe, fast and easy-to-use research device that allows 3D samples to be analyzed using the in situ hybridization, transparency of samples and immunohistochemistry methods. Thanks to this equipment, it is possible to directly visualize the location and activation of genes of the cells concerned. The means used for this localization is in situ hybridization (HIS) of RNA probes (transcriptome analysis). It should be noted that the automaton also allows proteome analysis by immunohistochemistry, using antibodies instead of RNA probes.