Immunohistochemistry (IHC)

Immunohistochemistry (IHC) is a method of locating proteins (antigens) in the cells of a tissue section or sample by detecting antigens with antibodies.

This technique exploits specific Antigen/Antibody bonds in biological tissues. Antibodies can be of polyclonal or monoclonal origin, monoclonal antibodies being more specific in nature.

IHC can be applied in many different ways by using different probes and developers. Indeed, primary antibodies can be coupled with substrate reactive enzymes, or coupled to fluorophores and visualized under an epifluorescence microscope. This last variation of IHC is often referred to as immunofluorescence.

To finely analyze the location of gene activation zones, the use of Antibody and IHC probes is very important. However, these manual techniques are difficult to implement, particularly on in toto samples such as a tissue fragment or animal embryo.

Thanks to the Flo400, the samples are subjected to a permanent flow of solvents that guarantees the transfer of the solution to the nuclei of the cells. A constant supply of reagent avoids any stagnation and preserves the integrity of biological samples.
The constant recycling of solvents, RNA probes and antibodies considerably reduces the analysis time. The solutions collected at the end of the session can be reused for further analysis.

Fields of application : Developmental biology, plant biology, dermo-cosmetics, oncology, neurology, toxicology.

The Flo400 PLC can also perform the techniques:

Research in biology is constantly diversifying and becoming more complex with the emergence of new technologies and the accumulation of knowledge. Thus, different fields of applications and experiments in biology are emerging and new high throughput biology techniques are emerging.

In situ hybridization, immunohistochemistry (IHC) and fluorescence in situ hybridization are tagging methods that provide a clear understanding of the expression of each gene in a spatial and temporal context within a given sample. This allows a characterization of the proteome or genome of the organism studied.

Flogentec thus offers biological automatons that can have several different applications depending on your research field.